There are a number of national primary roads in the county including the N5 road connecting Westport with Dublin, the N17 road connecting the county with Galway and Sligo and the N26 road connecting Ballina with Dublin via the N5. There are a number of national secondary roads in the county also including the N58 road, N59 road, N60 road, N83 road and N84 road.

Mayo is well served by rail travel. Westport railway station is the terminus station on the Dublin to Westport Rail service. Railway stations are also located at Ballyhaunis, Claremorris, Castlebar, Manulla, Ballina and Foxford. All railway stations are located on the same railway line, with exception to Ballina and Foxford which requires passengers to change at Manulla Junction. There are currently four services each way every day on the line.

There are also proposals to reopen the currently disused Western Railway Corridor connecting Limerick with Sligo.

Ireland West Airport Knock is an international airport located in the county. The name is derived from the nearby village of Knock. Recent years have seen the airports passenger numbers grow to over 650,000 yearly with a number of UK and European destinations.